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KMID : 0606920080160020100
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Biomolecules & Therapeutics
2008 Volume.16 No. 2 p.100 ~ p.105
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Dihydroceramide was Highly Elevated by the Fumonisin B₁ and Desipramine in Sphingomonas chungbukensis
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Burenjargal Munkhtsatsral
Yoo Hwan-Soo
Lee Youn-Sun
Yoo Jae-Myung
Lee Yong-Moon
Oh Sei-Kwan
Yun Yeo-Pyo
Kim Young-Chang
Choi Mi-Hwa
Ji So-Young
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Abstract
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The sphingolipid metabolites act as lipid mediator for cell proliferation and apoptosis in mammalian cells. In bacteria, sphingolipid metabolism remains unknown. The purpose of this study was to investigate whether sphingolipid metabolism is potential target for fumonisin () and desipramine in Sphingomonas chungbukensis, Gram-negative bacteria, by comparing the intracellular contents of bacterial sphingolipids with ones of HIT-T15 -cells, hamster pancreatic cells. The concentrations of ceramide and dihydroceramide were 18.0 12.0 and 0.025 0.018 nmol/mg protein, respectively, in HIT-T15 cells. However, the concentrations of ceramide and dihydroceramide in the bacterial culture were 2.0 1.2 and 10.6 5.5 nmol/mg protein, respectively. decreased the level of ceramide from 18.0 to 3.8 nmol/mg protein in HIT-T15 -cells. However, dihydroceramide content in -treated HIT-T15 cells was slightly decreased compared with the control culture. When S. chungbukensis was treated with either or desipramine, dihydroceramide level was increased by 5- and 4-fold, respectively, compared with the control bacteria. These results indicate that and desipramine may act as an activator in bacterial sphingolipid biosynthetic pathway, and bacterial sphingolipid metabolism pathway appears to be different from the pathway of mammalian cells.
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KEYWORD
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Dihydroceramide, fumonisin, desipramine, sphingolipid, Sphingomonas chungbukensis
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